A novel mechanism of protamine expression deregulation highlighted by abnormal protamine transcript retention in infertile human males with sperm protamine deficiency
نویسندگان
چکیده
منابع مشابه
A novel mechanism of protamine expression deregulation highlighted by abnormal protamine transcript retention in infertile human males with sperm protamine deficiency.
Sperm protamine deficiency has been associated with human male infertility. However, the aetiology of deregulated protamine expression remains elusive. The objective of this study was to evaluate the underlying aetiology of protamine deficiency in male infertility patients with deregulated protamine expression. Protamine-1 (P1) and protamine-2 (P2) protein concentrations were compared against P...
متن کاملChromomycin A3 Staining Indicates Sperm Protamine Deficiency
Chromomycin A3 binds to DNA and Protamine -deficient spermatozoa look bright yellow,whereas normal spermatozoa appear dull yellow.
متن کاملProtamine 2 precursors, protamine 1/protamine 2 ratio, DNA integrity and other sperm parameters in infertile patients.
BACKGROUND The protamine 1-to-protamine 2 ratio (P1/P2) is altered in the sperm cells of some infertile patients. Also, evidence for increased protamine 2 precursors (pre-P2) in a few patients has been reported. But so far, there have been no studies measuring simultaneously these two variables in a large number of patients. METHODS We measured the P1/P2 ratio and the presence of pre-P2 using...
متن کاملDecreased protamine-1 transcript levels in testes from infertile men.
Infertile men exhibit an aberrant protamine-1 (Prm1) to protamine-2 (Prm2) ratio at both the mRNA and protein level. We therefore investigated whether male infertility could be related to the amount of Prm1 and Prm2 mRNA by applying real time quantitative PCR following RNA extraction from routinely Bouin-fixed and paraffin-embedded testicular biopsies. Samples (n = 51) were normalized to the sa...
متن کاملEvaluation of sperm protamine deficiency and apoptosis in infertile men with idiopathic teratozoospermia
OBJECTIVE Sperm morphology plays an important role in infertility, especially in cases of defects in the heads of spermatozoa. Tapered-head or elongated-head spermatozoa are examples of morphological abnormalities. The aim of this study was to compare the semen parameters, levels of protamine deficiency, and frequency of apoptosis between patients with normozoospermia and those with teratozoosp...
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ژورنال
عنوان ژورنال: MHR: Basic science of reproductive medicine
سال: 2006
ISSN: 1460-2407,1360-9947
DOI: 10.1093/molehr/gah258